• Mashup Score: 1

    1. Always promotes the mission of the Knight Cardiovascular Institute through our Professional Standards of timely Customer Service, Reliability, Productivity, Professionalism, Accuracy, Timeliness, Integrity, and Problem-Solving.   2. Responsible for the lifecycle of bio samples while in the CTU from collection to shipping. This includes but is not limited to: – Works in collaboration with study coordinator to maintain and file regulatory and study documents of all samples collected for various studies. – Keeps detailed accounting records of all samples processed, shipped, and stored. – Creates and maintains a database of all bio samples in cryostorage. – Processes samples after collection and prepares for shipping. – Prepare, finalize, and deliver all packages for shipment at days end. – Maintaining laboratory reporting and signature requests from the PI . 3. Benchtop research for investigator initiated clinical trials, including but not limited to:  – Perform various molecular techn

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  • Mashup Score: 10

    BACKGROUND: RBM20 (RNA binding motif protein 20) cardiomyopathy is a severe form of dilated cardiomyopathy (DCM). Genetic variants in the nuclear localization signal of Rbm20 hinder its nuclear import and promote cytoplasmic pathogenic RNP (ribonucleoprotein) granules. We aimed to investigate whether reducing RNP granules by inhibiting Rbm20 expression could alleviate the DCM phenotype in Rbm20 S639G (Rbm20S639G) knock-in mice. METHODS: We downregulated Rbm20, utilizing antisense oligonucleotides (ASOs) that specifically inhibit Rbm20 expression. We administered Rbm20-ASOs in Rbm20S639G mice that carry a serine-to-glycine substitution in the nuclear localization signal of RBM20. The Rbm20-ASOs were administered subcutaneously at 25 mg/kg once a week for 8 weeks in both young (14-day-old) mice before the onset of DCM and adult (3-month-old) mice with established DCM phenotype. In vivo cardiac function was assessed by echocardiography. RNP granules were identified through fluorescent imm

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    • This is an elegant study --> an ASO that decreases sarcoplasic RBM20 granules, to prevent and rescue the DCM phenotype. A treatment approach that doesn't require underlying gene/splicing correction https://t.co/GUiPKewdah #CardioTwitter https://t.co/d70xvNjkvq