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Mashup Score: 0Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source - 3 year(s) ago
Author summary The field of genetic control of mosquito vectors has progressed rapidly in recent years, especially in Cas9-based control systems, due to its robustness to elicit a species-specific and dispersive control of mosquito population. To generate a Cas9-based integration, Cas9 and sgRNA are used to cleave a chromosomal locus while a plasmid DNA donor, containing a genetic cargo flanked…
Source: journals.plos.orgCategories: Genetics, Latest HeadlinesTweet
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Mashup Score: 1Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source - 3 year(s) ago
Author summary The field of genetic control of mosquito vectors has progressed rapidly in recent years, especially in Cas9-based control systems, due to its robustness to elicit a species-specific and dispersive control of mosquito population. To generate a Cas9-based integration, Cas9 and sgRNA are used to cleave a chromosomal locus while a plasmid DNA donor, containing a genetic cargo flanked…
Source: journals.plos.orgCategories: Genetics, Latest HeadlinesTweet-
How can we improve #CRISPR #Cas9 genetic editing of mosquitos? Ang et al assess the effects of sequence mismatches and template type on homology-directed repair to guide future designs @AEstadea @michell56085624 @AlpheyLab #mosquito #genetics #genomics https://t.co/V5JFoeFean
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Mashup Score: 1High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome - 4 year(s) ago
Author summary Germlines have evolved specialized mechanisms to protect themselves from invasions by transposons and viruses, which create barriers to genome editing techniques. For example, transgenes are silenced in the germline of the nematode C. elegans, thereby creating a barrier to CRISPR editing by Cas9. To facilitate gene editing, we built a collection of C. elegans strains in which Cas9…
Source: journals.plos.orgCategories: Genetics, Latest HeadlinesTweet-
Germline silencing of plasmid-borne #Cas9 causes inefficient editing in C. elegans Schwartz et al present a set of C. elegans strains that constitutively express Cas9 in the #germline from an integrated transgene, with a 50-100% editing rate #CRISPR https://t.co/zY7XrmlHZO
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Mashup Score: 1
CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. How…
Source: www.sciencedirect.comCategories: Genetics, Latest HeadlinesTweet-
This #openaccess paper from 2015 is still Journal of Biotechnology's Most Downloaded Article: Rapid and highly efficient mammalian cell engineering via #Cas9 protein transfection https://t.co/worgrpAOlA https://t.co/ErSzvobadX
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Mashup Score: 3
Original Article from The New England Journal of Medicine — CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis
Source: New England Journal of MedicineCategories: Hem/Oncs, Latest HeadlinesTweet-
👉👉👉Incredibly important paper this week in @NEJM || #CRISPR-#Cas9 In Vivo Gene Editing for Transthyretin #Amyloidosis | NEJM #TTR | #leusm #lymsm #mmsm https://t.co/JZiqWJihmg
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Mashup Score: 15Efficient embryonic homozygous gene conversion via RAD51-enhanced interhomolog repair - 4 year(s) ago
Co-injection of mouse zygotes with RAD51 and Cas9 can enhance interhomolog repair and facilitate conversion of heterozygous alleles to homozygous alleles.
Source: CellCategories: General Medicine Journals and Societies, Latest HeadlinesTweet-
Out now! Co-injecting zygotes with RAD51 and Cas9 enhances interhomolog repair and facilitates conversion of heterozygous alleles to homozygous alleles #embryo #Cas9 @ScienceMIT @MIT https://t.co/5um65xBAkP https://t.co/PmrhTMVQvU
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Mashup Score: 0Interrogating immune cells and cancer with CRISPR-Cas9 - 4 year(s) ago
CRISPR-Cas9 technologies have transformed the study of genetic pathways governing cellular differentiation and function. Recent advances have adapted these methods to immune cells, which has accelerated the pace of functional genomics in immunology and enabled new avenues for the design of cellular immunotherapies for cancer. In this review, we summarize recent developments in CRISPR-Cas9…
Source: Trends in ImmunologyCategories: Hem/Oncs, Latest HeadlinesTweet-
SCI member @Satpathology and Frank Buquicchio summarize recent developments in #CRISPR #Cas9 technology and discuss how they have been leveraged to discover and manipulate novel genetic regulators of the immune system. https://t.co/FsBTSqL8NF
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Mashup Score: 29Efficient embryonic homozygous gene conversion via RAD51-enhanced interhomolog repair - 4 year(s) ago
Co-injection of mouse zygotes with RAD51 and Cas9 can enhance interhomolog repair and facilitate conversion of heterozygous alleles to homozygous alleles.
Source: CellCategories: General Medicine Journals and Societies, Latest HeadlinesTweet-
Co-injecting zygotes with RAD51 and Cas9 enhances interhomolog repair and facilitates conversion of heterozygous alleles to homozygous alleles #embryo #Cas9 https://t.co/7ez1NOg150 https://t.co/jsacGXy962
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Mashup Score: 19Recording of elapsed time and temporal information about biological events using Cas9 - 4 year(s) ago
Indel generation by Cas9 and guide RNA can be used to record time and temporal information about biological events.
Source: CellCategories: General Medicine Journals and Societies, Latest HeadlinesTweet-
Indel generation by #Cas9 can be used to record #time and #temporal information about #biological events. https://t.co/6oAw32bfku @yonsei_u https://t.co/QNyX6s4qmX
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Mashup Score: 3Issue: Cell - 4 year(s) ago
StoriesStill we riseOlufolakemi “Fola” Olusanya is the winner of the first Rising Black Scientist Award for an undergraduate scholar. For this award, we asked emerging Black scientists to tell us about the experiences that sparked their journey in the life sciences. This is her story.Full-Text HTMLPDF Transforming myself and academia for goodChrystal StarbirdDr. Chrystal Starbird…
Source: www.cell.comCategories: General Medicine Journals and Societies, Latest HeadlinesTweet-
The new issue is out! Featuring insights into the water-to-land transition, gut #microbiome protection from #pathogenic #fungi, visualization of the #nuclear translocation of HIV-1 capsids, and using #Cas9 to record #biological events https://t.co/VDwrvlXe0J https://t.co/qMwaQU0kuk
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How can we improve #CRISPR genetic editing of mosquitos? Ang et al assess the effects of sequence mismatches and template type on homology-directed repair to guide future designs #mosquito #genetics #genomics #Cas9 #CRISPRCas9 https://t.co/V5JFoeFean