Programmable genome engineering and gene modifications for plant biodesign

This review examines programmable genome engineering and modification strategies for plant biodesign, with an emphasis on modular systems that enable multidimensional control over genetic, transcriptional, and epigenetic states. It traces the progression of DNA-targeting technologies from protein-based to RNA-guided platforms, discusses the versatility of effector domains for dynamic gene regulation, and highlights inducible systems that confer spatiotemporal control. The review also outlines applications in decoding gene networks, engineering ideal optimizedcrops, and advancing plant synthetic biology.

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