Efficacy of Combined Use of PELNAC and Platelet-rich Plasma … : Plastic and Reconstructive Surgery – Global Open
ined the underlying mechanism. Methods: Two 1-cm² full-thickness skin defects were created in 8-week-old male nude rats (n = 8). One defect received saline-impregnated PELNAC (control group); the other received PRP-impregnated PELNAC (combination group). Cytokine levels in PRP were measured. Wound area measurements and histological and immunohistochemical evaluations (transforming growth factor-β1 [TGF-β1], vascular endothelial growth factor [VEGF], α-smooth muscle actin, and von Willebrand factor) were performed on day 14. Results: Compared with the control group, the combination group showed a significantly reduced wound area (32.4 ± 14.89 versus 68.3 ± 14.9 mm², P < 0.001) and increased dermis-like granulation tissue thickness (947 ± 219 versus 448 ± 165 μm, P < 0.001). Expression of TGF-β1 (8.9% ± 1.7% versus 4.2% ± 1.2%, P < 0.0001) and VEGF (4.4% ± 1.2% versus 1.9% ± 0.5%, P < 0.0001) was significantly higher in the combination group, whereas α-smooth muscle actin–positive cells
